FIG 4.

Overexpression of TPK1 and EFG1 restores hyphal growth and virulence defects of a CDC3-DAmP mutant strain. (A) CDC3-DAmP (JRB412), CDC12-DAmP (JRB418), and septin DAmP strains overexpressing TPK1 were spotted onto spider agar plates and incubated at 37°C for 6 days. Images of the edges of representative single colonies are shown. (B) A marker-matched wild-type strain (DAY185) and CDC3-DAmP (JRB412), CDC3-DAmP+CDC3 (JRB421), and CDC3-DAmP+TPK1-OE (JRB473) strains were injected into the tail veins of 8 mice each. Survival of infected strains was recorded daily. The time to death was significantly delayed for mice infected with the CDC3-DAmP strain (P = 0.017) but was not significantly different in the mice infected with the wild-type, CDC3-DAmP+CDC3 (P = 0.18), or CDC3-DAmP+TPK1-OE (P = 1.0) strain.