TgCRT-containing vesicles change their morphology in extracellular parasites. Immunofluorescence assays of extracellular parasites expressing TgCRT-HA3, colabeled with antibodies against TgV-ATP (A), TgVP1, a marker for the PLV (B), TgNHE3, a second marker for the PLV (C), and TgCPL, a marker for the VAC (D). Scale bars, 3 μm.