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Loss of TgCRT results in swelling of the VAC. (A) Representative images of wild-type (RH) and iTgCRT/ΔTgCRT parasites grown in the absence and presence of ATc and labeled with anti-CPL antibodies to highlight the VAC. (B) Quantification of VAC diameters in wild-type and iTgCRT/ΔTgCRT parasites grown in the presence and absence of ATc. ****, P < 0.0001; n.s., not significant.
