FIG 1.

Sites of bud emergence. (A) Zygotes can bud either from the midzone (medial [M]), along the flanking surfaces (lateral [L]), or toward their ends (terminal [T]). The sum of L and T is referred to as “nonmedial.” (B) Landmarks needed for initial bud site specification. Homotypic crosses were conducted, fixed, and counted. Averages of the bud distributions (medial, lateral, and terminal) from at least three independent experiments are plotted in each case. The dashed horizontal line indicates the control value for medial budding as a point of reference. Quantitation is given in Table S2 in the supplemental material. Note that most budding is medial unless Rax1 has been deleted. (We were unable to examine axl1Δ crosses because Axl1 is required for mating. Since rax2Δ strains are prohibitively petite and therefore likely to be genetically unstable, we have not explored the possible significance of the protein.)