TABLE 1.

Monosaccharide composition of N. gaditana cell walls determined after enzymatic digestion

Enzyme	% of wall mass solubilized (mean ± 1 SD; n = 3)a	% of mass (mean ± 1 SD; n = 3) determined to be carbohydrates by:		% of glucose in carbohydratesb,c	Other carbohydrates detected
		HPAEC-PADb	Anthrone
Cellulase 1	76.0 ± 2.5d	81.4 ± 8.3d	78.3 ± 5.6d	98.9d	Fuc, Man, Rha, GalA
	83.5 ± 2.2e	82.9 ± 3.7e	78.9 ± 9.3e	98.0e
	77.4 ± 2.5f	73.3 ± 2.6f	68.7 ± 1.4f	99.0f
Cellulase 2	71.5 ± 2.0	70.1 ± 2.0	NDg	97.2	Fuc, Gal, GalA
Chitinase	69.6 ± 6.3	64.3 ± 1.7	ND	98.9	Fuc, GlcN, Rha, GalA
Chitosanase	11.3 ± 1.3	11.6 ± 0.5	ND	100.0	None
Lyticase	68.9 ± 3.5	65.4 ± 4.1	ND	99.2	Fuc, Rha, GalA
Protease	9.9 ± 6.2	1.1 ± 0.2	ND	28.5	Fuc, GlcN, Man, Rha, GalA
Zymolyase	71.0 ± 2.2	71.7 ± 3.5	ND	99.9	Fuc, GlcN

^aThe percentage of the cell wall mass that was solubilized was determined gravimetrically.

^bCarbohydrates as the percentage of total cell wall mass and glucose as the percentage of total carbohydrate were determined by HPAEC-PAD after digestion of the enzyme hydrolysate with 2 M TFA at 100°C for 6 h. Carbohydrate values reflect the amounts after subtraction of carbohydrates measured in wall-free controls.

^cStandard deviations were all <0.05%.

^dGrown in f/2 medium: this sample was used for all digestions using alternate enzymes.

^eGrown in f/2 medium.

^fGrown in SW medium.

^gND, not determined.