CreMIP1 is localized at the CV membrane in Chlamydomonas reinhardtii. Ultrastructure of Chlamydomonas reinhardtii. (A) Longitudinal section; (B) cross-section through the two CV regions of a cell. (C to E) Immunogold localization of CreMIP1 at the CV membranes using 10-nm gold particles. BB, basal body; C, chloroplast; CV, contractile vacuole; ER, endoplasmic reticulum; G, Golgi complex; LV, lytic vacuole; M, mitochondrion; N, nucleus; P, pyrenoid. Scale bars are 500 nm (A), 200 nm (B), and 100 nm (C to E).