FIG 7.

Expression of the aquaporin CreMIP1 in different strains of Chlamydomonas reinhardtii UVM4 expressing CreMIP1-GFP fusion protein. (A) Western blots. Whole-cell extracts of Chlamydomonas reinhardtii were probed with an α-GFP antibody and an α-Arf1 antibody (loading control; expected molecular mass, 20.59 kDa). L, protein ladder. (B) Endogenous CreMIP1 (expected molecular mass, 31.54 kDa) is present in cell extracts of all cell lines. (C) qPCR. The primer pair MIP1-CDS (CDS = coding sequence) detects all transcripts of MIP1 and MIP1-GFP, while the primer pair MIP1–3′UTR (UTR = untranslated region) detects only transcripts of the endogenous MIP1. Error bars indicate SEM.