TABLE 1.
Strains used or constructed in this study
| Strain | Genotype | Reference |
|---|---|---|
| S. cerevisiae BW31a | MATa leu2-3/122 ura3-1 trp1-1 his3-11/15 ade2-1 can1-100 GAL SUC2 mal10 ena1-4Δ::HIS3 nha1::LEU2 | 26 |
| Z. rouxii | ||
| CBS 732T | Wild type | CBS strain database |
| UL4 | ura3 | 19 |
| F1 | ura3 Zrffz1Δ::loxP | This study |
| F2 | ura3 Zrffz2Δ::loxP | This study |
| F1F2 | ura3 Zrffz1Δ::loxP Zrffz2Δ::loxP | This study |
| S1 | ura3 Zrfsy1Δ::loxP | 17 |
| F1F2S1 | ura3 Zrffz1Δ::loxP Zrffz2Δ::loxP Zrfsy1Δ::loxP | This study |
| F1F2x | ura3 Zrffz1Δ::loxP Zrffz2Δ::loxP | This study |
| F1F2(pZEU) | F1F2 + pZEU; Zrffz1Δ::loxP Zrffz2Δ::loxP | This study |
| F1F2(pZEU_ZrFFZ1) | F1F2 + pZEU_ZrFFZ1; Zrffz2Δ::loxP | This study |
| F1F2(pZCA_ZrFFZ1) | F1F2 + pZCA_ZrFFZ1; Zrffz2Δ::loxP | This study |
| F1F2(pZEU_ZrFFZ2) | F1F2 + pZEU_ZrFFZ2; Zrffz1Δ::loxP | This study |
| F1F2(pZGFP_ZrFFZ2) | F1F2 + pZGFP_ZrFFZ2; Zrffz1Δ::loxP | This study |
| F1F2(pZCA_ZrFFZ2) | F1F2 + pZCA_ZrFFZ2; Zrffz1Δ::loxP | This study |