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Expression of Ptr2 mutant proteins. 3HA-tagged wild-type or Ptr2 mutant proteins were expressed in ptr2Δ trp1 cells (A), PTR2 trp1 cells (B), or ptr2Δ TRP1 cells (C). Exponentially growing cells in SD medium were moved to Ala-Trp (AW) medium (20 μg · ml−1) and incubated for an additional 5 h. Whole-cell extracts were subjected to Western blot analysis to detect Ptr2-3HA and Adh1 using specific antibodies. Adh1 was used as a loading control.
