TABLE 1.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pJJ280 | TRP1 | 30 |
| YCplac33 | URA3 CEN4 | 31 |
| pUA35 | pTDH3-3HA-tTDH3, URA3 CEN4 | This study |
| pKK1 | GFP URA3 CEN4 | This study |
| pPTR2–3HA | PTR2-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E92Q-3HA | PTR2-E92Q-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-R93K-3HA | PTR2-R93K-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-Y96A-3HA | PTR2-Y96A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-Y138A-3HA | PTR2-Y138A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-D149E-3HA | PTR2-D149E-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G168A-3HA | PTR2-G168A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-L196A-3HA | PTR2-L196A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K205R-3HA | PTR2-K205R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-S209A-3HA | PTR2-S209A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F244L-3HA | PTR2-F244L-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F247L-3HA | PTR2-F247L-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-W362L-3HA | PTR2-W362L-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E480D-3HA | PTR2-E480D-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G487A-3HA | PTR2-G487A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-L488A-3HA | PTR2-L488A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-M504L-3HA | PTR2-M504L-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F507W-3HA | PTR2-F507W-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-T510V-3HA | PTR2-T510V-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G514A-3HA | PTR2-G514A-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K16R-3HA | PTR2-K16R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K27R-3HA | PTR2-K27R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K34R-3HA | PTR2-K34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K77R-3HA | PTR2-K77R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2–3K>R-3HAa | PTR2-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2–4K>R-3HA | PTR2-K16,27,34,77R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E92Q-3K>R-3HA | PTR2-E92Q-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-R93K-3K>R-3HA | PTR2-R93K-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K205R-3K>R-3HA | PTR2-K205R-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-W362L-3K>R-3HA | PTR2-W362L-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E480D-3K>R-3HA | PTR2-E480D-K16,27,34R-3HA driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-GFP | PTR2-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E92Q-GFP | PTR2-E92Q-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-R93K-GFP | PTR2-R93K-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-Y96A-GFP | PTR2-Y96A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-Y138A-GFP | PTR2-Y138A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-D149E-GFP | PTR2-D149E-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G168A-GFP | PTR2-G168A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-L196A-GFP | PTR2-L196A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-K205R-GFP | PTR2-K205R-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-S209A-GFP | PTR2-S209A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F244L-GFP | PTR2-F244L-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F247L-GFP | PTR2-F247L-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-W362L-GFP | PTR2-W362L-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-E480D-GFP | PTR2-E480D-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G487A-GFP | PTR2-G487A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-L488A-GFP | PTR2-L488A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-M504L-GFP | PTR2-M504L-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-F507W-GFP | PTR2-F507W-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-T510V-GFP | PTR2-T510V-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2-G514A-GFP | PTR2-G514A-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2–3K>R-GFP | PTR2-K16,27,34R-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
| pPTR2–4K>R-GFP | PTR2-K16,27,34,77R-GFP driven by PTR2 promoter, URA3 CEN4 | This study |
a
K>R, K-to-R substitution.