TABLE 2.
Sequences of the primers used in this study
| Primer sequence (5′–3′) | Gene and locationa |
|---|---|
| GACAAAATGAAGAAAATGCTGATGCACC | POL1 ORF (F) |
| TAATAACCTTGGTAAAACACCCTG | POL1 ORF (R) |
| GCTGAGTTTAACGGTGATTATT | TEL VI-R (F) |
| CCAGTCCTCATTTCCATCAAT | TEL VI-R (R) |
| ACGGCTCTCCAGTCATTTAT | ARG1 UAS (F) |
| GCAGTCATCAATCTGATCCA | ARG1 UAS (R) |
| TAATCTGAGCAGTTGCGAGA | ARG1 TATA (F) |
| ATGTTCCTTATCGCTGCACA | ARG1 TATA (R) |
| TGGCTTATTCTGGTGGTTTAG | ARG1 5′ORF (F) |
| ATCCACACAAACGAACTTGCA | ARG1 5′ORF (R) |
| TTCTGGGCAGATCTACAAAGA | ARG1 3′ORF (F) |
| AAGTCAACTCTTCACCTTTGG | ARG1 3′ORF (R) |
| TATACGGCCTTCCTTCCAGTT | ADH1 CORE (F) |
| TGTGCAGCAAAAGAAACAAGGA | ADH1 CORE (R) |
| CCCACGGTAAGTTGGAATACA | ADH1 5′ORF (F) |
| TGACCACCGACTAATGGTAGC | ADH1 5′ORF (R) |
| CTTACGTCGGTAACAGAGCTGA | ADH1 3′ORF (F) |
| ACCAACGATTTGACCCTTTTC | ADH1 3′ORF (R) |
| CGACGACGAAGACAGTGATA | PMA1 5′ORF (F) |
| ATTCTTTTTCGTCAGCCATTG | PMA1 5′ORF (R) |
| TGTTTTGGGTGGTTTCTACTACG | PMA1 3′ORF (F) |
| TTAGGTTTCCTTTTCGTGTTAGAG | PMA1 3′ORF (R) |
| AAATAGGCACAAACACGACC | STE11 3′ORF (F) |
| ATTATGTGTGCATCCAGCCA | STE11 3′ORF (R) |
| GCTGATGAAACCTCTGCATCTAC | FLO8 3′ORF (F) |
| CAACCATACCAATATTCCCAA | FLO8 3′ORF (R) |
| GAAACAAGAACTGGAAGATGCCGAAGAGGAGAGGAAATTGATGATGGCAGAAGCCCGTGCAAAGAGACGGATCCCCGGGTTAATTAA | SPT6Δ202-Myc tagging (F) |
| TTTGTAATGGTTTTAAGACGCTTCTAAAATCTAACAGTAGTAAGAATAGAATGAACAACTACCGTCGGATCCCCGGGTTAATTAA | Spt6-Myc tagging (F) |
| CTTACCTAAACAATGGTCAAAGTAATAATAAAATTAATAATAACAATGGACACTACATACGCATGAATTCGAGCTCGTTTAAAC | Spt6-Myc tagging (R) |
a
F and R, forward and reverse primer sequences, respectively.