FIG 4.
Teb1C requirements for DNA binding and telomerase activation involve distinct domain surfaces. (A) Schematic of Teb1C variants used in this study. The L45 loop and α-helix at the C terminus were added to the crystal structure (35) by modeling using Chimera (49). (B) SDS-PAGE of bacterially expressed and purified His6-Teb1BC proteins. (C and D) Calculated Kd values for each of the Teb1BC proteins as determined by EMSA in triplicate (C), with a representative EMSA (D). (E) Activity assay of recombinant Teb1BC reconstitution of holoenzyme catalytic activity.