FIG 5.

Inducible expression and holoenzyme assembly of FZZ-tagged Teb1 variants reveal requirements for Teb1 holoenzyme assembly. (A and B) Western blots of cells from full-length Teb1-FZZ transgene expression strains with single-residue substitutions of the A, B, or C domain (A) or other Teb1C domain substitutions or deletions (B). (C) Western blot of representative Teb1-FZZ expression across the time course of cell cycle analysis. (D and E) Telomerase activity assay following FLAG antibody purification of Teb1-FZZ complexes from cell extracts.