FIG 2.

LID-1 is a homolog of CGI-58 and localizes to lipid droplets. (A) Phylogenic trees of CGI-58 gene family members on the basis of amino acid sequences from Homo sapiens (Hs), Mus musculus (Mm), Danio rerio (Dr), and Caenorhabditis elegans (Ce). Amino acid sequences encoded by all genes were subjected to multiple alignments and analyzed for phylogenetic trees obtained by the neighbor-joining method using ClustalW. (B) Alignment of the GXSXG motif in the α/β hydrolase domain of CGI-58 gene family. (C) Homology scores of LID-1 (C25A1.12) obtained from the Homologene database. (D) Confocal images showing the localization of GFP-fused LID-1 in live worms and in fixed worms costained with Oil Red O and Nile red. The stained area of the Oil Red O image is pseudocolored red. The insets are magnified views. Scale bars, 10 μm. White lines indicate the boundaries of worm bodies. (E) Lysophosphatidyl choline acyltransferase activity assay. One-microgram quantities of total bacterial protein and purified recombinant proteins were used.