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. 2014 Nov;34(22):4165–4176. doi: 10.1128/MCB.00722-14

FIG 3.

FIG 3

LID-1, a C. elegans homolog of CGI-58, is required for ATGL-1 activity. (A and B) Representative images and quantitation data of Oil Red O staining with or without lid-1 RNAi in young adult worms under feeding or fasting conditions. Marked areas were subjected to quantitation of Oil Red O staining. (C and D) Representative images and quantitation data of Oil Red O staining in atgl-1(hj67) worms after L4440 control and lid-1 RNAi. Marked areas were subjected to quantitation of Oil Red O staining. (E) Coimmunoprecipitation (IP) assay of HEK293T cells expressing ATGL-1-Flag and LID-1-Myc. (F) Confocal images of atgl-1(hj67) worms under feeding and fasting conditions after L4440 control or lid-1 RNAi. Scale bars, 10 μm. White lines indicate the boundaries of worm bodies. Error bars represent standard deviations. **, P < 0.01.