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. 2014 Nov;34(22):4165–4176. doi: 10.1128/MCB.00722-14

FIG 4.

FIG 4

PKA signaling mediates fat mobilization upon fasting in C. elegans. (A and B) Representative images and quantitation data of Oil Red O staining with or without kin-1 RNAi in young adult worms under feeding or fasting conditions. (C) Cyclic AMP (cAMP) concentrations measured by direct ELISA in total extracts of wild-type worms under feeding and 4-h-fasting conditions. (D and E) Representative images and quantitation data of Oil Red O staining in wild-type and AMPK mutant worms under feeding or fasting conditions. (F and G) Representative images and quantitation of Oil Red O intensities with control, aak-1, or aak-2 RNAi in young adult worms under feeding or fasting conditions. (H and I) Representative images and quantitation data of Oil Red O staining in the PKA hyperactive kin-2 mutant strain (ce179 strain) after RNAi of atgl-1 or lid-1. For Oil Red O staining, marked areas were subjected to quantitation. Error bars represent standard deviations. *, P < 0.05; **, P < 0.01.