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. 2014 Nov;34(22):4165–4176. doi: 10.1128/MCB.00722-14

FIG 6.

FIG 6

PKA increases ATGL-1 protein stability and LID-1 binding. (A) Western blot data of C. elegans ATGL-1 proteins in Cos-1 cells treated with cycloheximide (CHX; 50 μg/ml). Forskolin (50 μM) was pretreated for 3 h. GAPDH was used as a loading control. (B) Confocal images of atgl-1(hj67) worms treated with DMSO, forskolin (100 μM), or MG132 (100 μM) under feeding or fasting conditions. (C) Ubiquitination assay of ATGL-1 in Cos-1 cells. (D) Coimmunoprecipitation assay of Cos-1 cells expressing ATGL-1-Flag and LID-1-Myc in the absence or presence of forskolin (50 μM, 3 h). (E) Confocal images of atgl-1(hj67) worms after L4440 control and kin-1 RNAi under feeding or 4-h-fasting conditions. Scale bars, 20 μm. White lines indicate the boundaries of worm bodies.