FIG 2.

Downregulation of miR-34a increased mitochondrial function and expression of UCP1 in all types of WATs and BAT. BALB/c mice were treated as described in the legend to Fig. 1. (A) Immunostaining of UCP1 by IHC. (B) Mitochondrial DNA copy numbers. mDNA and MtDNA, mitochondrial DNA; nDNA, nuclear DNA. (C) Mitochondrial citrate synthase activities (n = 3). *, P < 0.05; **, P < 0.01. (D to G) mRNA levels of genes involved in thermogenesis and lipid metabolism as determined by qRT-PCR. Data are means and SEM (n = 5). *, P < 0.05; **, P < 0.01; ***, P < 0.001.