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Regulation of PHO89 expression by Snf1 may be mediated by Mig2. (A) Wild-type strain DBY746 and its reg1 and reg1 snf1 derivatives were transformed with pPHO89-LacZ and subjected to high-pH stress (pH 8.0) (black bars) prior determination of β-galactosidase activity. (B) pPHO89-LacZ activity was measured in wild-type strain DBY746 and in strains containing different combinations of the snf1, mig1, and mig2 mutations. Data are means ± standard errors of the means from 9 to 12 experiments.
