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Snf1 and Rim101 control PHO89 expression through Mig2 and Nrg1. The indicated strains were transformed with plasmid pPHO89-LacZ. Exponential cultures were switched to pH 8.0, and β-galactosidase activity was determined after 90 min. Data are means ± standard errors of the means from 12 to 15 experiments (A) or 20 experiments (B).
