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. 2014 Dec;34(24):4379–4388. doi: 10.1128/MCB.00740-14

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FIG 3 — Atg9A bears a conserved canonical 14-3-3ζ binding phosphomotif. (A) HA-Atg9A was overexpressed in HEK-293 cells, and cells were treated with hypoxia for the indicated times. Immunoprecipitated HA-Atg9A was immunoblotted with an anti-14-3-3ζ phosphomotif antibody (pMotif) and for endogenous 14-3-3ζ. (B) Sequence alignment of Atg9A sequences from different species. Highlighted letters indicate the conserved canonical 14-3-3ζ binding motif. (C) Consensus logo for a canonical 14-3-3ζ binding motif generated by aligning 60 verified 14-3-3 binding motifs using WebLogo (http://weblogo.berkeley.edu/).