FIG 2.
Wheel-running activities and cellular rhythms of CRY2(S557A) knock-in mice. (A) Wheel-running activity records for WT (CRY2+/+) and homozygous mutant (CRY2S557A/S557A) mice are shown in a double-plot format. Animals entrained to LD cycles were transferred to DD. Asterisks in the actogram indicate the time point when a 30-min light pulse was given. (B) Periodogram estimates of the period for each genotype. Data for the 7th to 21st days in DD were used to calculate the circadian period. The circadian periods were plotted as means with standard errors of the means. The number of animals is indicated in parentheses (*, P < 0.05 by Student's t test). (C) Phase shifts in response to a 30-min light pulse at CT14 or CT22 (n.s., nonsignificant difference). (D) PER2::LUC bioluminescence rhythms of mouse liver slices. For detrended data, we subtracted the averaged bioluminescence signals for 24 h. (E) Circadian periods of the liver from the 4th peak to the 6th peak (means with standard errors of the means; n = 8) (*, P < 0.05 by Student's t test).