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. 2014 Dec;34(23):4343–4354. doi: 10.1128/MCB.00533-14

FIG 5.

FIG 5

Defective accumulation of CD3+ CD4+ T cells in cutaneous sites of secondary Ag challenge in WIPΔABD mice. (A) Proliferation and secretion of IL-2, IFN-γ, and IL-13 in response to OVA by splenocytes from WIPΔABD mice and WT controls EC-immunized with OVA (n = 5 for each group). (B) Representative FACS analysis of CD3+ CD4+ cells in ears of WIPΔABD mice and WT controls 7 days following i.d. challenge with OVA323-339 peptide or PBS as a control. (C) Percentages and numbers of CD3+ CD4+ cells in ears of WIPΔABD mice and WT controls 7 days after i.d. challenge with OVA323-339 peptide or PBS as a control (n = 5 for each group). (D) Ifnγ and Il13 mRNA expression in the ears of WIPΔABD mice and WT controls 7 days after i.d. challenge with OVA323-339 peptide or PBS as a control. The data are represented as the fold increase in mRNA levels relative to PBS-challenged ears of WT mice set at 1 (n = 5 for each group). n.d., not detected. Columns and bars represent means ± the SEM. ***, P < 0.001; **, P < 0.01; *, P < 0.05; n.s., not significant.