FIG 5.

Accumulation of editing intermediates in RNAi knockdowns correlates with placement of respective subunits within RESC assemblies. Data represent the results of Northern blotting of preedited, partially edited (46 editing events at the 5′ region were not completed and 107 events in the preceding region were completed), and fully edited RPS12 mRNAs. Total RNA was separated on a 5% polyacrylamide–8 M urea gel and hybridized with single-stranded DNA probes. Cytosolic rRNA (5.8S) was used as a loading control.