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Binding of recombinant rhesus (Rh) macaque FH domains 6 and 7 to immobilized FHbp, as measured by ELISA. Fifty nanograms of each of the recombinant FH fragments fused to mouse IgG2a Fc was added to the wells, and binding was detected with anti-mouse IgG conjugated to alkaline phosphatase. As a positive control, we used human (Hu) FH 6,7/Fc containing Y352 in domain 6. As a negative control, we used human FH 18–20/Fc.
