FIG 3.

Functional characterization of T cells stimulated by the HIVconsv vaccines. BALB/c mice were vaccinated by the ChAdV63.HIVconsv-MVA.HIVconsv regimen. One week after MVA.HIVconsv, oligofunctional profiles of HIVconsv-elicited T cells were assessed by ICS assay. (A) Representative FACS plots for TNF-α or CD107a versus IFN-γ in response to peptides H, 42, and 112. (B) Frequencies of CD8⁺ T cells expressing TNF-α, CD107a, or IFN-γ upon stimulation using either peptide pools P1 to P6 (left) or individual peptides 42, 112, and H (right). Data are represented as means ± standard errors of the means (n = 3). (C) Oligofunctional profiles of CD8⁺ T cells specific for peptide pools P2, P4, and P6. Data are represented as means (n = 3). The intragroup variations were low as shown in panel B and were not plotted for this analysis.