FIG 4.

Cytotoxic potential of peptide 42-, 112-, and H-specific T cells induced by the HIVconsv vaccines. BALB/c mice were immunized with ChAdV63.HIVconsv followed by MVA.HIVconsv as shown in Fig. 2A. One week after MVA.HIVconsv, the splenocytes were tested for their potential to kill peptide-pulsed P815 cell targets. (A) Representative FACS plots showing the gating strategy. SSC, side scatter; FSC, forward scatter. (B) Percent specific lysis of peptide-pulsed P815 target cells at an effector-to-target ratio of 10:1 (left panel) or 5:1 (right panel). The horizontal lines represent the medians.