Plasma cytokine and chemokine levels in mice infected with Ad5 vectors. Mice were infected with either Ad5-null, Ad5-VCA0956, or Ad5-VCA0956*. At 6 and 24 h, cytokines and chemokines were quantified from plasma samples. Brackets indicate statistical significance, which was determined using a two-way ANOVA test combined with a Bonferroni posttest (*, P < 0.05, and **, P < 0.01). Bars indicate the means from independent mouse plasma samples (n = 2: mock, Ad5-null; n = 3: Ad5-VCA0956 and Ad5-VCA0956*), and error bars indicate standard deviations.