FIG 1.

Validation of filovirus microarrays using control antibodies. A panel of antibodies against Marburg virus (A), Zaire ebolavirus (B), and Sudan ebolavirus (C) proteins were tested on printed microarrays. All antibodies are mouse monoclonal except for anti-Zaire NP and VP40, which are both rabbit polyclonal. Bound antibodies were detected fluorescently on a microarray scanner. Background-corrected fluorescence intensities were averaged across technical replicates on the microarrays. The bars represent mean fluorescence (relative fluorescence units [RFU]) ± standard error of the mean (SEM). All GP ΔTMs were expressed in insect cells except for Marburg GP ΔTM (Musoke), which was expressed in mammalian cells. B, Bundibugyo; T, Tai Forest; M, Marburg; R, Reston; S, Sudan; Z, Zaire.