FIG 2.

IgG antibody responses detected using filovirus microarrays. Naive, postimmunization (immunized), and postviral challenge (challenged) sera from Zaire ebolavirus (A) and Marburg virus (B) animal studies were applied to the assembled microarrays. Bound IgG antibodies were detected fluorescently on a microarray scanner. Following data preprocessing, normalized fluorescence signals were averaged across the five animals in each study. The bars represent normalized mean fluorescence (RFU) ± SEM. The cutoff line represents 2 standard deviations above the mean antibody signal observed in the naive sera. For each antigen-antibody response, paired t tests were done for naive versus immunized and naive versus challenged sera. Unless indicated with an asterisk, all immunized and challenged samples above the cutoff line were found to have significant antibody increases (P < 0.05) in comparison with the naive samples. (C) Side-by-side comparison of GP-specific IgG signals in challenged sera from Zaire ebolavirus and Marburg marburgvirus studies.