FIG 2.

Adenoviral detection and E2 antigen characterization in milk samples subjected to viral inactivation. Adenoviral titers were determined by transduction in HEK-293A cells using samples from goat milk before and after treatment with methylene blue (50 μM), used as viral inactivation agent. Positive standard deviation bars are shown. Statistically significant differences (P < 0.01) between treated and untreated samples at 48 h posttransduction are also indicated. Inset, Western blot analysis of E2 antigen present in goat milk samples after the methylene blue viral inactivation treatment. Lane 1, milk serum corresponding to day 1 posttransduction, in which samples were taken from udders but no inactivation treatment was conducted. Lane 2, milk serum treated with methylene blue. Lane c−, milk serum from a nontransduced goat (negative control). Lane c+, E2 from PK-15 adenovirally transduced cells.