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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Adv Exp Med Biol. 2014;825:159–197. doi: 10.1007/978-1-4939-1221-6_5

Figure 1. Systems biology approaches applied to study Piwi pathways.

Figure 1

Four main biochemical techniques now enable system-wide analysis of the Piwi-piRNA pathway. Cross-Linking Immuno Preciptiation (CLIP) enriches transcripts associated with Piwi proteins. Chromatin ImmunoPrecipitation (ChIP) enriches genomic DNA regulated by Piwi complexes. Global Run On (GRO) and Nascent RNA analyses reinforced a transcriptional gene silencing role by Piwi, while messenger RNA and piRNA analyses were facilitated by high-throughput deep sequencing of cDNA libraries.