FIG 3.
CA of in vitro transcription from the ilv-leu promoter. The in vitro transcription was performed as described in the text. In vitro transcription with the DNA template carrying Pilv-leu (nt −246/+26) and part of lacZ produced a 250-base runoff transcript. The lanes (1, 1/2, 1/4, and 1/8) contained stepwise-diluted CcpA and P-Ser-HPr solutions, whereas lane 0 contained neither CcpA nor P-Ser-HPr; the most right-hand lane 1 (no dilution) contained CcpA (3.0 μM as a dimer) and P-Ser-HPr (3.7 μM).