FIG 4.

Helix face-dependent interaction of the complex of CcpA and P-Ser-HPr with RNAP for CA of ilv-leu. (A) Strains FU676, FU846, FU849, FU847, and FU848 carried lacZ fusions with the ilv-leu promoter region (−248/+26) possessing Δ0 (no deletion), Δ11, Δ16, Δ21, and Δ32, respectively, whose deletion centers were at nt −61 inside CodY-I+II. Strains FU735, FU861, FU864, FU862, and FU863 carry Δ0, Δ11, Δ16, Δ21, and Δ32, respectively, in the ΔcodY genetic background. The CodY-binding motif is shown at the bottom. (B) The cells of these strains were grown to an OD₆₀₀ of 0.5 under AA-rich conditions, and then β-Gal activity in crude cell extracts was measured and plotted.