. 2014 Nov;196(21):3724–3734. doi: 10.1128/JB.01933-14

TABLE 1.

Strains and primers used in this study

Strain or primer	Description or sequence (5′–3′)	Source^a and/or reference	Location
Strains
CPM7	CP1250 SsbB⁻::pEVP3::SsbB⁺; SsbB⁺; Sm^r Cm^r	13
CP1250	Rx1 hex malM511 str-1 bgl-1; low-α-galactosidase background; Sm^r	1
CP1344	CP1250 ΔclpC::PcTet; Sm^r Tc^r	18
CP1376	CP1250 comW::kan; Sm^r Kn^r	9
CP1500	Rx1 hex nov-r1 bry-r str-1 ery-r1 ery-r2; Nv^r Em^r Sm^r	21
CP1759	CP1250 comW::Spc; Spc^r Sm^r	12
CP2000	CP1250 Δcps; Sm^r	21
CP2107	CP2000 Δcps SsbB⁻::pEVP3::SsbB⁺; SsbB⁺; Sm^r Cm^r	CP2000 × CPM7 (21)
CP2136	CP2107 comA::Cheshire; Sm^r Cm^r Em^r	35
CP2137	CP2000 ΔCheshire comA mutant; Sm^r Cm^r	35
CP2451	CP2137 rpoD-L363F; Sm^r Cm^r	CP2137 × NYT1 {rpoD}
CP2452	CP2137 rpoD-A171V; Sm^r Cm^r	CP2137 × ALT4 {rpoD}
CP2453	CP2137 rpoD-R355H; Sm^r Cm^r	CP2137 × FLT4 {rpoD}
CP2454	CP2137 rpoD-R316H; Sm^r Cm^r	CP2137 × ILT1 {rpoD}
CP2455	CP2451 ΔcomW::kan; Sm^r Cm^r Kn^r	CP2451 × CP1376 {ΔcomW::kan}
CP2456	CP2452 ΔcomW::kan; Sm^r Cm^r Kn^r	CP2452 × CP1376 {ΔcomW::kan}
CP2457	CP2453 ΔcomW::kan; Sm^r Cm^r Kn^r	CP2453 × CP1376 {ΔcomW::kan}
CP2458	CP2454 ΔcomW::kan; Sm^r Cm^r Kn^r	CP2454 × CP1376 {ΔcomW::kan}
CP2459	ALT4 rpoD-V171; Sm^r Cm^r Kn^r Nv^r Em^r Tc^r	ALT4 × CP2137 {rpoD}
CP2460	FLT4 rpoD-H355; Sm^r Cm^r Kn^r Nv^r Em^r Tc^r	FLT4 × CP2137 {rpoD}
CP2461	ILT1 rpoD-H316; Sm^r Cm^r Kn^r Nv^r Em^r Tc^r	ILT4 × CP2137 {rpoD}
CP2462	NYT1 rpoD-F363; Sm^r Cm^r Kn^r Nv^r Em^r Tc^r	NYT1 × CP2137 {rpoD}
CP2463	CP2137 ΔcomW::kan; Sm^r Cm^r Kn^r	CP2137 × CP1376 {ΔcomW::kan}
ALT4	CP2463 rpoD-A171V; Sm^r Cm^r Nv^r Em^r Tc^r	Spontaneous rpoD mutation^b
FLT4	CP2463 rpoD-R355H; Sm^r Cm^r Nv^r Em^r Tc^r	Spontaneous rpoD mutation^b
ILT1	CP2463 rpoD-R316H; Sm^r Cm^r Nv^r Em^r Tc^r	Spontaneous rpoD mutation^b
NYT1	CP2463 rpoD-L363F; Sm^r Cm^r Nv^r Em^r Tc^r	Spontaneous rpoD mutation^b
Primers
DAM497	CAATTGACTATATTAGAGGCGAGACA		spr0016
DAM500	TATCAAGCGCATCATTCAAGATAACAG		purA
YT18	GCCCTAGAAGAATTGGAACG		dnaG
YT20	CAGGGTCGATGACTTCTTCC		spr0980
YT30	GACAGGCTTTGAGTCTCTTGATGG		spr0977
YT31	CGGACGCTCAAACTTGGCTAATTC		spr0982
YT34	CCATTGCCAAACGCTATGTC		rpoD
YT36	GTCAACCGCCTTCATCAAGCC		rpoD
YT40	TATGGGCTTGATGAAGGC		rpoD
YT41	TATGGGCTTGATGAAGGT		rpoD
YT42	ATACGCTCACGAGTTACG		rpoD
YT43	CGTGAAGAAAATGTTCTGCG		rpoD
YT44	CGTGAAGAAAATGTTCTGCA		rpoD
YT45	TCACATCTGCCTCGATTG		cpoA
YT46	TTGCTACGACTTGGTTGGC		rpoD
YT47	TTGCTACGACTTGGTTGGT		rpoD
YT48	GTCAATGACCCTGTCCGTATG		cpoA
YT49	CAAGTCGTAGCAAACCGC		rpoD
YT50	CAAGTCGTAGCAAACCGT		rpoD
YT51	CACGGTAAGCACCTGAAAC		cpoA
YT76	AGCGCCGACAGGGATTGGGA		dinG
YT77	ACATTGGCCTTTTGACGTGCAT		ezrA

Braces indicate transfer of the gene only via the PCR amplicon. ×, transformation cross as recipient X donor.

Followed by transformation with nov-r1, ery-r1 ery-r2, and ΔclpC::PcTet donors.