TABLE 3.
σA substitutions in bypass mutants
| Independent enrichmenta | Individualb | Relative efficiencyc | σA substitutiond |
|---|---|---|---|
| ALT | 5 | 0.002 | R297C |
| 2 | 0.007 | R316C | |
| 1 | 0.01 | P287S | |
| 3 | 0.02 | A171V | |
| 4 | 0.05 | A171V | |
| DET | 1 | 0.0003 | − |
| 3 | 0.006 | A171V | |
| 4 | 0.009 | − | |
| 5 | 0.05 | A171V | |
| FLT | 3 | 0.004 | R355H |
| 5 | 0.006 | R355H | |
| 1 | 0.03 | R316H | |
| 4 | 0.08 | R355H | |
| GAT | 5 | 0.00008 | − |
| 4 | 0.02 | R316H | |
| 2 | 0.06 | R316H | |
| 3 | 0.08 | A171V | |
| 1 | 0.09 | R316H | |
| ILT | 3 | 0.0004 | − |
| 4 | 0.1 | R316H | |
| 2 | 0.26 | R316H | |
| 1 | 0.3 | R316H | |
| 5 | 0.31 | R316H | |
| MAT | 2 | 0.002 | − |
| 1 | 0.003 | − | |
| 4 | 0.003 | Y290C | |
| 3 | 0.01 | R316H | |
| 5 | 0.02 | A171V | |
| MTT | 4 | 0.008 | R316H |
| 3 | 0.03 | A171T | |
| 2 | 0.06 | A171V | |
| 1 | 0.07 | R316H | |
| 5 | 0.07 | R316H | |
| NCT | 1 | 0.001 | − |
| 3 | 0.02 | A171V | |
| 5 | 0.02 | A171V | |
| 2 | 0.07 | A171V | |
| 4 | 0.07 | A171V | |
| NVT | 2 | 0.003 | V314A |
| 4 | 0.02 | A171V | |
| 5 | 0.02 | A171V | |
| 3 | 0.07 | V314A | |
| 1 | 0.1 | R316H | |
| NYT | 5 | 0.00008 | − |
| 4 | 0.008 | L363F | |
| 1 | 0.18 | L363F | |
| 3 | 0.28 | R316H | |
| 2 | 0.41 | R316H | |
| OHT | 1 | 0.05 | R355H |
| PRT | 2 | 0.0009 | − |
| 1 | 0.003 | − | |
| 3 | 0.04 | R355H | |
| 4 | 0.1 | R316H | |
| 5 | 0.1 | R316H | |
| 6 | 0.2 | R316H | |
| SCT | 5 | 0.08 | R316H |
| 4 | 0.1 | R316H | |
| 2 | 0.13 | R316H | |
| TXT | 2 | 0.06 | R316H |
| 5 | 0.1 | R316H | |
| 4 | 0.11 | R316H | |
| WT | 1 | WT | |
| ΔcomW | 0.0001 | WT |
a
Name of enrichment series.
b
Tcr clone number.
c
Ratio of mutant transformation efficiency to that of the WT. The SD values of quadruplicate measurements were below 20%.
d
Boldface, mutation identified by WGS; −, isolate not sequenced.