FIG 5.

Detection of 837pep1 and 837pep2 by Western blotting using an anti-FLAG antibody. The FLAG-tagged strains, Pep1-C-FLAG (left) and Pep2-C-FLAG (right), were grown exponentially and total cell lysates were generated as described in Materials and Methods. Proteins from clarified whole-cell lysates were separated by SDS-PAGE in a 12% acrylamide gel. The experiment was performed twice with similar results. See the text for details on which cellular fractions contained detectable FLAG-tagged peptides. WT, wild type.