TABLE 2.

Amino acid changes in lytically functional single-Cys alleles and triggering times of the alleles

Amino acid position	Amino acid residue or changea	Triggering time (min)b
7	H7C	55
TMD1 positions
12	A12C	35
13	I13C	30
14	L14C	35
15	A15C	20
16	A16C	20
17	K17C	75
20	G20C	30
21	I21C	20
23	A23C	75
24	I24C	35
27	F27C	75
28	A28C	20
TMD2 positions
45	V45C	30
49	T48C	60
51	C51 (parental)	35
53	I53C	45
54	I54C	65
55	A55C	45
57	F57C	50
TMD3 positions
68	S68C	55
69	S69C	30
71	L71C	70
72	A72C	50
74	I74C	55
75	T75C	40
77	V77C	45
78	F78C	40
79	I79C	70
80	G80C	55
81	Y81C	75
95	A95C	30
108	Stop108C	30

^aAll changes are missense changes, and all are in the context of the C51S substitution.

^bTriggering time in minutes after induction.