Skip to main content
. 2014 Dec 1;7:91. doi: 10.3389/fnmol.2014.00091

Figure 1.

Figure 1

Expression pattern of clathrin in transgenic zebrafish. (A) Confocal image of the eye of a Ribeye:clathrin-EGFP larva (i, 5 dpf). Clathrin LCa-EGFP is expressed densely in the synaptic terminals of bipolar cells in the inner plexiform layer (ii, IPL). The right-hand panel is an example of a bipolar cell dissociated from such a fish (iii). (B) Western Blot with a primary antibody directed against clathrin neuronal light chain. Protein bands at appropriate molecular weights were labeled in membrane homogenates of retinae from mouse, goldfish, wild-type zebrafish, Ribeye::ClathrinLCa-EGFP (RCE) zebrafish and Ribeye::synaptophysin-EGFP (RSE) zebrafish. The antibody against clathrin LCa recognizes a doublet at about 36–38 KDa in the mouse retina and a triple band in goldfish retina (Sherry and Heidelberger, 2005). In wild-type zebrafish and RSE retinae, the antibody recognizes two species at about 34–36 KDa. In RCE retinae a third band is detected is detectable at about 70 KDa, corresponding to the clathrin LCa-EGFP fusion protein (indicated by the black arrow). (C) Immunostaining of dissociated bipolar cells. (i) Distribution of Clathrin LCa-EGFP is similar to the endogenous protein (red). Clathrin LCa-EGFP co-localized with synaptophysin 1 (ii) and with SV2 (iii) Scale bars: 2 μm.