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. 2014 Dec 1;7:91. doi: 10.3389/fnmol.2014.00091

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Copyright © 2014 Pelassa, Zhao, Pasche, Odermatt and Lagnado.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic model. Clathrin is immobilized on docked-vesicles and endosomes/cisternae at the active zone. When a vesicle fuses we observe a clear SypHy signal (green trace) but no net signal of clathrin (red), because the net recruitment and accumulation occurs while the sypHy signal is already decaying (red trace). During the uptake of the vesicle we observe a decay of clathrin signal indicating that these areas were remote from sites of synaptic vesicle fusion and retrieval. With TIRF imaging we were able to discriminate at different hotspots all steps of vesicle recycling.