FIG 2.
R. albus chromosomal region encoding the electron-bifurcating ferredoxin- and NAD-dependent [FeFe]-hydrogenase HydABC and the region on plasmid pRuma101 encoding the ferredoxin-dependent [FeFe]-hydrogenase HydA2 and putative H2-sensing [FeFe]-hydrogenase HydS. The cofactor content was predicted from the amino acid sequence of the proteins. RT-PCR analysis revealed that the genes for HydA, HydA2, and HydS are expressed during growth of R. albus 7 in batch culture on glucose. gDNA, genomic DNA; RNA, total RNA. The numbers in the scheme correspond to numbers in Rumal_3402 to Rumal_3408 locus tags.