TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description or purpose | Reference or source |
|---|---|---|
| Serratia sp. strains | ||
| SCBI | Wild type | 3 |
| 16-D2 | Transposon mutant with disrupted aprE | This study |
| 22-A7 | Transposon mutant with disrupted bglB | This study |
| 22-B9 | Transposon mutant with disrupted inosine/xanthosine triphosphatase | This study |
| 22-C7 | Transposon mutant with disrupted gidA | This study |
| 22-D7 | Transposon mutant with disrupted MCP | This study |
| 22-F6 | Transposon mutant with disrupted PIN protein | This study |
| E. coli strains | ||
| S17-1 λpir | Tpr Smr recA thi pro hsdS(r− m+) RP4::2-Tc::Mu::Km Tn7 λpir; donor strain for transposon mutagenesis | 64 |
| DH5α λpir | supE44 ΔlacU169 (ϕlacZΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1; λpir phage lysogen; host strain for gene complementation | 65 |
| Plasmids | ||
| pMiniHimar RB1 | Transposon mutagenesis | 35 |
| pCR2.1-TOPO | TOPO cloning | Invitrogen |
| pBAD33-Cm | Expression vector | 37 |
| pBAD33-AprE | pBAD33 vector containing cloned aprE plus tolC | This study |
| pBAD33-GidA | pBAD33 vector containing cloned gidA | This study |
| pBAD33-MCP | pBAD33 vector containing cloned gene (SCBI_0077) coding for MCP | This study |
| pBAD33-PIN | pBAD33 vector containing cloned gene (SCBI_0044) coding for the PIN protein | This study |