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. 2014 Nov;196(22):3923–3936. doi: 10.1128/JB.01908-14

TABLE 2.

Genetic complementation of mutants 16-D2, 22-C7, 22-D7, and 22-F6 restored wild-type phenotypes

Straina Radius (mm) of the clearing zone on skim milk agarb,e Protease activity azocasein assay (U/109 cells)c,e Insect pathogenesis LT50 value (no. of days)d,e
WT Serratia sp. strain SCBI 8.1 ± 0.2 11.42 ± 0.57 2.96
WT SCBI + pBAD33 7.7 ± 0.3 10.86 ± 0.43 3.08
WT SCBI + pBAD33-AprE 8.3 ± 0.2 11.59 ± 0.98 3.08
WT SCBI + pBAD33-GidA 8.2 ± 0.1 11.98 ± 0.47 2.87
WT SCBI + pBAD33-MCP 8.1 ± 0.2 11.31 ± 0.67 2.96
WT SCBI + pBAD33-PIN 8.3 ± 0.2 10.90 ± 0.47 3.21
Mutant 16-D2 0.5 ± 0.0* 0.25 ± 0.05* 1.48*
Mutant 16-D2 + pBAD33 0.5 ± 0.1* 0.38 ± 0.06* 1.61*
Mutant 16-D2 + pBAD33-AprE 7.1 ± 0.4 10.12 ± 1.76 2.75
Mutant 22-C7 1.0 ± 0.1* 1.24 ± 0.27* 8.35*
Mutant 22-C7 + pBAD33 1.0 ± 0.2* 1.36 ± 0.57* 8.49*
Mutant 22-C7 + pBAD33-GidA 6.9 ± 0.4* 9.21 ± 2.13 3.54
Mutant 22-D7 1.0 ± 0.2* 1.52 ± 0.04* 5.29*
Mutant 22-D7 + pBAD33 1.1 ± 0.1* 1.59 ± 0.05* 5.05*
Mutant 22-D7 + pBAD33-MCP 7.5 ± 0.3 9.74 ± 2.03 3.67
Mutant 22-F6 1.2 ± 0.2* 1.69 ± 0.02* 5.22*
Mutant 22-F6 + pBAD33 1.2 ± 0.2* 1.56 ± 0.01* 4.90*
Mutant 22-F6 + pBAD33-PIN 7.8 ± 0.5 10.35 ± 1.67 3.21
a

Wild-type (WT) or mutant bacterial strain alone or carrying plasmid pBAD33 or derivatives of pBAD33.

b

Skim milk agar plates were spot inoculated with strains that had been grown for 18 h at 28°C in LB medium. After 48 h of incubation, the radius of the clearing zones were measured. These values were expressed in millimeters and are the averages of four measurements. The standard deviations are from at least two independent experiments.

c

Proteolytic activity was determined using an azocasein assay and performed with strains grown for 18 h at 28°C in LB medium. Values are averages of three measurements ± standard deviations from at least two independent experiments.

d

LT50 values represent the average time (in days) for 50% of the M. sexta larval population to die.

e

Values that are significantly different (P < 0.05) from the value for wild-type Serratia sp. SCBI are shown with an asterisk.