FIG 10.
Influence of rex inactivation on transcript levels of the genes involved in detoxification of ROS and molecular O2 in C. acetobutylicum. Total RNA was isolated from the wild-type, rex-inactivated mutant, and rex-complemented strains grown in P2 minimal medium and harvested at an OD600 of about 2.0. The expression levels of each gene were normalized to the gene expression in the wild-type strain. Data represent means ± standard deviations of values from six measurements starting from two independent cultures. Differences in the mRNA levels of all the studied genes between the wild type and rex-inactivated mutant are statistically significant (P < 0.01).