FIG 5.

Effect of NADH and NAD⁺ on the DNA-binding activity of Rex. (A) EMSAs were performed using C. acetobutylicum adhE2 promoter fragment (1 nM), Rex protein (60 nM), and the indicated concentrations of pyridine nucleotides. No protein was added to the first lane. (B) EMSAs were performed as in panel A but with a range of physiological concentrations of NADH and NAD⁺.