TABLE 2.

Comparison of mRNA levels in C. acetobutylicum wild type and rex-inactivated mutant using qRT-PCR

Gene name	Gene identifier	mRNA ratioa (rex mutant/WTb)
adhE2	CAP0035	164.75 ± 6.45
ldh	CAC0267	13.75 ± 0.84
thlA	CAC2873	12.46 ± 2.34
crt	CAC2712	2.43 ± 0.10
bcd	CAC2711	2.81 ± 0.78
etfB	CAC2710	2.17 ± 0.20
etfA	CAC2709	1.55 ± 0.14
hbd	CAC2708	2.79 ± 0.37
ptb	CAC3076	6.07 ± 1.35
buk	CAC3075	1.97 ± 0.12
nadA	CAC1025	2.53 ± 0.38
nadB	CAC1024	1.79 ± 0.12
nadC	CAC1023	1.79 ± 0.26
asrT	CAC1512	2.04 ± 0.72
asrA	CAC1513	2.41 ± 0.74
asrB	CAC1514	2.67 ± 0.36
asrC	CAC1515	1.69 ± 0.48

^aData represent means ± standard deviations of values of mRNA ratios obtained from six measurements starting from two independent cultures. The strains were cultivated in P2 minimal medium, and total RNA was isolated in the exponential growth phase at an OD₆₀₀ of about 2.0. The P value of the mRNA ratios for all the genes studied is smaller than 0.01.

^bWT, wild type.