TABLE 2.
Consumption of substrates and production of fermentation products during the batch cultivation in ASW-YT-Pyr
| Strain | OD600 | Consumption (mmol/liter of culture) |
Production (mmol/liter of culture) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pyruvate | Amino acidsa | NH3 | Acetate | Alanine | Acidsb | Formate | CO2 | H2 | NH3 | Biomassc | ||
| KUW1 | 1.12 ± 0.08 | 37.0 ± 0.3 | 10.2 ± 1.0 | 4.50 ± 0.07 | 24.8 ± 1.1 | 20.4 ± 0.4 | 7.5 ± 0.2 | 0.97 ± 0.13 | 21.5 ± 1.8 | 14.7 ± 1.0 | 3.14 ± 0.22 | |
| KUW1Δhyh | 1.51 ± 0.09 | 32.9 ± 0.01 | 17.0 ± 0.1 | 31.7 ± 1.8 | 14.4 ± 1.6 | 11.5 ± 0.3 | 0.18 ± 0.02 | 33.4 ± 1.9 | 33.6 ± 1.9 | 8.01 ± 0.40 | 4.24 ± 0.26 | |
| KUW1ΔΔhyh_pfl | 1.54 ± 0.05 | 32.8 ± 0.4 | 16.0 ± 1.9 | 32.5 ± 1.8 | 15.4 ± 0.4 | 12.7 ± 0.01 | 0.22 ± 0.03 | 34.1 ± 4.0 | 33.9 ± 2.7 | 8.24 ± 0.68 | 4.31 ± 0.13 | |
| KUW1ΔΔhyh_por | 0.73 ± 0.02 | 22.9 ± 0.2 | 21.4 ± 0.6 | 8.3 ± 0.1 | 23.4 ± 0.7 | 16.7 ± 0.6 | 0.01 ± 0.00 | 17.8 ± 1.4 | 18.3 ± 0.6 | 5.84 ± 0.29 | 2.05 ± 0.07 | |
| KUW1ΔΔhyh_vor | 1.19 ± 0.08 | 32.3 ± 0.1 | 9.4 ± 0.3 | 36.6 ± 0.2 | 8.9 ± 0.1 | 7.2 ± 0.1 | 0.15 ± 0.02 | 33.9 ± 2.2 | 34.0 ± 2.5 | 7.69 ± 0.04 | 3.34 ± 0.23 | |
a
Trp consumption is estimated from indoleacetate (derived from Trp) production. Ala is not included in the sum.
b
The amount includes carboxylic acids corresponding to amino acids, except for formate and acetate. Thioglycolate (derived from Cys), 5-aminovalerate (Lys), 4-guanidinobutanoate (Arg), and imidazoleacetate (His) production was estimated from the corresponding amino acid consumption.
c
Based on the assumption that the organic fraction of cells is C5H7NO2, and this comprises 90% of dry cell weight (11).