FIG 5.

Regulation of slrP by lon, leuO, and hilD at the protein level. The level of SlrP was studied by immunoblotting using strains expressing a 3×FLAG fusion. Extracts from stationary-phase cultures of these strains were resolved by SDS-PAGE (12%), and a monoclonal anti-FLAG antibody was used for Western blotting (upper bands). Polyclonal anti-GroEL or anti-DnaK antibodies were used as a loading control (lower bands). Molecular mass markers are indicated on the left. c, wild-type background. (A) Effects of overexpression and deletion of hilD on slrP expression. (B) Effect of Lon on slrP expression in the presence and in the absence of HilD. (C) Effect of LeuO on slrP expression in the presence and in the absence of HilD.