FIG 1.

Effect of cbb₃ oxidases on anaerobic growth and cell morphology. Anoxic incubation of P. aeruginosa was performed using Hungate tubes containing argon gas and LBN medium at 37°C with shaking at 200 rpm. (A) Time course of OD measurements. Three independent experiments were carried out, and representative data are shown. (B) Transcriptional activities of two cbb₃ oxidases. ccoN promoter-dependent C23O activities in cells possessing pMEXccoN1 or pMEXccoN2 were measured and normalized to the C23O activities in cells possessing pMEX9. The values represent the means ± standard deviations from three independent experiments. NS, not significant. *, P < 0.05 (unpaired two-tailed Student's t test). (C) Time course of DIC images. Three independent experiments were carried out, and representative images are shown.