FIG 5.

Effect of cbb₃ oxidases on the cellular NO level. (A) Relative cellular NO levels at 6 h as determined by a NO detection reagent, DAF-2 DA. The values represent the means ± standard deviations from three independent experiments. *, P < 0.05 (unpaired two-tailed Student's t test). (B) Relative ODs and the amounts of NO₂⁻ remaining for the ΔccoN1 ΔccoN2 mutant at 14 h in LBN medium supplemented with 1 μM NO donor SNP and/or 2 mM NO scavenger PTIO. The values represent the means ± standard deviations from three independent experiments. NS, not significant. **, P < 0.01 (unpaired two-tailed Student's t test). (C) DIC images at 14 h. Three independent experiments were carried out, and representative images are shown.